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The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system

After all tests were completed, spleen and brain were collected for further analysis

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The membranes were incubated with 5% BSA at room temperature for 1 h, then with primary antibodies at 4 C overnight and second antibody successively for 1 h at room temperature, and consequently developed on the imaging system after adding ECL reagent

This has resulted in lively discussions amongst medical professionals and the public about whether this was indeed the nurses fault