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2.5 Western blot (WB) analysis Total protein was extracted from frozen cortical and hippocampal tissues using RIPA lysis buffer (Beyotime, cat#P0013B) containing a protease inhibitor cocktail (Beyotime cat#P1006)

However, similar to what we observed for strep-MBP-GRXC2, strep-MBP-HsGRX1 became oxidized already upon addition of GSH, resulting in two main protein species which persisted over a wide range of GSH/GSSG ratios (Fig

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Additionally, for patients with acquired hypothalamic obesity due to hypothalamic injury (such as craniopharyngioma and its treatment), where the pathogenesis is complex and GLP-1 therapy offers limited benefits, no approved drugs are currently available