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Immortalized BMDMs were maintained in RPMI 1640 with 2 mM L-glutamine, 1 penicillinstreptomycin (GenClone), 10% (v/v) heat-inactivated FBS (R&D Systems) and 20% conditioned medium

The filtered eluent containing intestinal cells was subjected to two rounds of centrifugation at 120 g for 3 min each cycle in DMEM containing 2% sorbitol

92 , E520E528 (2017)

All vials freeze-dried, independently tested, UK same-day dispatch

Overall, Melanotan 1 acts by mimicking the natural hormone a-MSH to stimulate melanogenesis and promote skin pigmentation, while potentially having additional effects on inflammation and cellular responses to UV radiation

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